Endodermal Development and Germ Cell Tumors – Role of Gata Transcription Factors
نویسندگان
چکیده
.................................................................................................................................... 6 Original Publications ............................................................................................................... 8 Abbreviations............................................................................................................................ 9 Introduction ............................................................................................................................ 10 Review of the Literature ........................................................................................................ 12 1. Endoderm ..................................................................................................................... 12 Primitive endoderm – the yolk sac................................................................................ 12 Definitive endoderm derivatives................................................................................... 14 Regulators of endodermal differentiation ..................................................................... 17 2. Germ cell tumors.......................................................................................................... 21 Normal germ cell development and tumor formation................................................... 21 Clinical aspects ............................................................................................................. 24 3. GATA transcription factors ......................................................................................... 26 Overview....................................................................................................................... 26 Hematopoietic GATA factors ....................................................................................... 27 GATA factors expressed in endodermal tissues ........................................................... 28 Friends of GATA .......................................................................................................... 30 GATA factors of non-vertebrates ................................................................................. 31 GATA factors in human disease ................................................................................... 31 Aims of the Study ................................................................................................................... 33 Materials and Methods .......................................................................................................... 34 1. Human tumors (I–III) ................................................................................................. 34 2. Human germ cell tumor cell lines (I).......................................................................... 35 3. Normal and transgenic mouse tissue (I, II, IV).......................................................... 35 4. mRNA in situ hybridization (I, II, IV) ........................................................................ 36 5. Semi-quantitative RT-PCR (VI) .................................................................................. 36 6. Northern blotting (I) .................................................................................................... 37 7. Immunohistochemistry (I–IV)..................................................................................... 37
منابع مشابه
I-51: The Role of the Transcription FactorGCNF in Germ Cell Differentiation and Reproductionin Mice
The germ cell nuclear factor (GCNF) is a member of the nuclear receptor super family of transcription factors. GCNF expression during gastrulation and neurulation is critical for normal embryogenesis in mice. GCNF represses expression of the POU domain transcription factor Oct4 during mouse post-implantation development in vivo. Oct4 is thus down-regulated during female gonadal development, whe...
متن کاملExpression of transcription factor GATA-4 during human testicular development and disease.
GATA-4 is a highly conserved transcription factor that plays a critical role in regulating embryonic morphogenesis and cellular differentiation. Given the emerging role of GATA-4 in the development and function of murine gonads, we have now studied its role in human testis. We find that GATA-4 is expressed from early human fetal testicular development to adulthood. This transcription factor is ...
متن کاملNotch-GATA synergy promotes endoderm-specific expression of ref-1 in C. elegans.
The Notch signaling pathway is involved in a wide variety of cell-fate decisions during development. The diverse behavior of Notch-activated cells is thought to depend on tissue- or cell-type-specific transcription factors, yet the identities of such factors and the mechanism of cooperation with the Notch pathway are largely unknown. We identify here an enhancer in the promoter of ref-1, a C. e...
متن کاملEnhanced cardiogenesis in embryonic stem cells overexpressing the GATA-4 transcription factor.
GATA-4 is a cardiac-specific member of the GATA family of zinc finger transcription factors. During embryogenesis, GATA-4 expression is detected very early in the cardiogenic area and persists later in the developing heart. Studies have shown that GATA-4 is a potent transcriptional activator of several cardiac muscle-specific genes and a key regulator of the cardiomyocyte gene program. Consiste...
متن کاملA two-step model for the fate determination of presumptive endodermal blastomeres in Xenopus embryos
BACKGROUND In Xenopus, the endoderm germ layer is derived from the vegetal blastomeres of cleavage-stage embryos. Cell transplantation experiments have revealed that the endodermal fate becomes gradually fixed during the late blastula stages. Sox17alpha, Mix.1, Mixer and GATA-4 encode vegetal zygotic transcription factors with endoderm-inducing activity. The accumulation of their transcripts du...
متن کامل